LOCUS       40924_30750        7052 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pMFXU4 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7052)
  AUTHORS   Komeda T, Tazumi K, Shimada H, Kano K, Hyashi T, Saito H, Tsumura H,
            Sakai Y, Kato N, Kondo K.
  TITLE     Production of Active Bovine Cathepsin C (Dipeptidyl Aminopeptidase 
            I) in the Methylotrophic Yeast Candida boidinii
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7052)
  AUTHORS   Komeda T, Kondo K, Sakai Y, Kato N.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-OCT-2001) Toshihiro Komeda, Central Laboratories for 
            Key Technology, Kirin Brewery Co., Ltd.; 1-13-5, Fukuura, 
            Kanazawa-ku, Yokohama, Kanagawa 236-0004, Japan 
            (E-mail:t-komeda@kirin.co.jp, Tel:81-45-788-7217, 
            Fax:81-45-788-4042)
REFERENCE   3  (bases 1 to 7052)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7052)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (11-OCT-2001) Toshihiro Komeda, Central Laboratories for Key 
            Technology, Kirin Brewery Co., Ltd."; volume: " 1-13-5, Fukuura, 
            Kanazawa-ku, Yokohama, Kanagawa 236-0004, Japan 
            (E-mail:t-komeda@kirin.co.jp, Tel:81-45-788-7217, Fax"; pages: 
            "81-45-788-4042"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7052
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      425..1600
                     /standard_name="mFDH1"
                     /label=mFDH1
                     /regulatory_class="promoter"
     misc_feature    1601
                     /label=NotI cloning site
                     /note="NotI cloning site"
     regulatory      1609..2237
                     /standard_name="FDH1"
                     /label=FDH1
                     /regulatory_class="terminator"
     CDS             complement(2891..3703)
                     /codon_start=1
                     /gene="URA3"
                     /product="orotidine-5'-phosphate decarboxylase"
                     /label=URA3
                     /note="Candida boidinii orotidine-5'-phosphate
                     decarboxylase"
                     /protein_id="BAE72068.1"
                     /translation="MSETLKTQPYSKRAEVHPSPVARRLFNLMETKKTNLCASVDLTTT
                     KEILDLLEKVGPYICLVKTHIDIVSDFSYEGTIVPLLALAKKYNFMIFEDRKFADIGNT
                     VKSQYKGGVYQIAKWSDITNAHGVTGSGIVTGLKQAAEEVTDEPRGLLMLAELSSKGAI
                     AHGKYTEETVEIAKTDKDFVIGFIAQNQMGGSDEGFDWIIMTPGVGLDDKGDALGQQYR
                     TVSEVMSTGTDIIIVGRGLFGKGRDPSVEGERYMKAGWNAYLARTNQL"
     gene            complement(2891..3703)
                     /gene="URA3"
                     /label=URA3
     primer_bind     complement(4831..4847)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(4855..4871)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4879..4909)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4924..4945)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(5233..5821)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5995..6852)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(6853..6957)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt accgggcccc
      421 tcgatcgagt ttaccactat ccaattaaaa tccatggatc agacggtagc tttaccacta
      481 tccaattaaa atccatggat cagacggtag tcgagtttac cactatccaa ttaaaatcca
      541 tggatcagac ggtagcttta ccactatcca attaaaatcc atggatcaga cggtagtcga
      601 gctgggtttt tactgaattc agtcaagtaa tcctgtcgga ctttttaata tctagctttc
      661 acaaaaacca acaacaacaa ccgctaatcc catcaaacaa ttaaacaatt gttacaattg
      721 tcacaattct tggatataca ataattaaac atacgtacat tcttacatac atatagagtt
      781 tgaaatagat acattaccca gtgtcatcga tattatgccc cgcctttttc acttgaaaca
      841 ataactatta ttactactat tattatttct attcatatat cctaaaaatt atattaaaat
      901 tggctctttt atgcaaaaaa tgtacattta tggtaatact agtcagatgt tataattata
      961 tctttaccac tatccaatta aaatccatgg atcagacggt agtttttata tctgtaacat
     1021 cttactacta ccactactac taccactact actaccacta ctactaccac tactactact
     1081 gataataagg tatactacat tttatcatac gtgaaatgta acgcgtagat taaacatttt
     1141 tttaaaatta ctgatcagta ctttccacaa taagcactta ttaatatgtg cctctttaaa
     1201 attacttaat tccctttact tttcattttt acaaccgctt tggtatttac ccccagagtg
     1261 ttttaattgc aattgaattc ttattttaat ttccattact ttctttgtac cataatgaaa
     1321 ttgccgagtt gtccctcctt tgaatttaaa tcattctcta atatttaact ttaattttaa
     1381 tattttagtt atttatttga attaaagtaa attcaactaa aaattgaact atttaaacac
     1441 tatgatttcc ttcaattata ttaaaatcaa tttcatattt ccttacttct ttttgcttta
     1501 ttatacatca ataactcaat taactcattg attatttgaa aaaaaaaaac atttattaac
     1561 ttaactcgag gattatatat tatattattg actttacaaa gcggccgcat tttcttaact
     1621 tgaaaactat aattgctata acaattcttc aatttctctt tttcttcctt tttttgaaga
     1681 atttttaaca atcaaaattt tgactctttg atttcccgca atctctgagc tcagcatact
     1741 cattattatt ttattattat tattattatt acttttatta ttattatatt ttttcttctt
     1801 taacgatatc gtttgtgttt tatcttttat gatttaaatt ttatacgaat ttatgaatac
     1861 aacaaaatat ttaagtttac acaatgtagt aaattaaaag ttaatcagta aaatgtattg
     1921 taagttacat atatcatata tcagtgtctt gatatatata aagaaaacgt tgcttatgta
     1981 atcaggcaca cgttgacagc gtggtgccct gaacagcaac cgcaattttg accacgcacg
     2041 ggcggtctta tctccagccg gctgctttca gtctccaagc gataaggcca caatagcaat
     2101 ggcaatcaca gccacagcca cgccagccat ctacttacgc tctctcttcc tgctgaggct
     2161 atcggtactc gcactactat tagcccttcc ctattcttcc tcttatcagc ttcatctctt
     2221 gagtcttacc ccatgcagca gcttcactat tggctattca atatcctata accaaagttc
     2281 aaaatataca tctttgcagg ttagaggcat tagatattta taatagagca gttaataatg
     2341 agcctagacg ttttttcaaa ttatactaca attcttatat tcaaacattt gatcaaatat
     2401 tagcaatgaa attaaaatct aaattggatt ggtggcaatt cttttataaa ggttttgtaa
     2461 ggaatgcatt agttaatatc cctgcaacat ctattgcttt attggttttt gaaattacta
     2521 gacaagaatt aagtaatgat attgaaagtt tgcttggtcc tacttgaagt cgatgtttcc
     2581 atttctatat tcatttaaat tatatttttt gtacgggaat gtgttttttt aaaaaaaaaa
     2641 ttcattctca ttctcattta tatacatatt attcacagaa atatatagag ttgtgtacta
     2701 tagagtccac tcaatacata gcgcttgtac attttattac tttgtttgct ttttattttt
     2761 agtaaattaa attcatattt ttattttttt gtaatatttt aaaactagaa ctatatatga
     2821 tatttaatat acagcgtaaa ataaattata gataaatcaa tcgattataa atgattgact
     2881 atatgatatt ttataattgg ttagttctag caagataagc attccaacct gctttcatgt
     2941 atctttcacc ttcaacagat gggtctctac ctttaccaaa taaacctcta ccgacaatga
     3001 taatatcagt acctgtggac ataacttctg agacagttct gtattgttgt cctaaagcat
     3061 cacctttatc atctaaacca acacctggag tcatgatgat ccaatcaaaa ccttcatctg
     3121 aaccacccat ttgattttga gcaatgaaac caataacaaa atctttatca gttttagcaa
     3181 tttcaacagt ttcttcagta tattttccat gggcaatggc accctttgat gataactcag
     3241 ctaacattaa taaacctctt ggttcatcag taacttcttc agctgcttgt tttaaaccgg
     3301 tgacaatacc tgaaccggtg acaccatgag catttgtaat atcagaccat ttggcaatct
     3361 gataaacacc acctttatat tgagatttga ctgtgttacc aatatctgca aattttctat
     3421 cttcaaaaat cataaagttg tattttttag caagggctaa aagtggaaca atagttcctt
     3481 cgtaactgaa atcagaaacg atatcgatat gagtcttgac taaacaaatg taaggaccaa
     3541 ctttttctaa taagtctaag atttctttgg ttgtggtgag atcaacagaa gcacataaat
     3601 tggtcttctt ggtttccatt aaattgaaca gacgacgagc gactggagaa ggatgaactt
     3661 cagctctctt ggagtatggt tgtgttttaa gagtttcaga cattgcagta aaattgtttg
     3721 ttgctggaaa atcagaaatg agctaaatga atgtaaaaca gatacaataa gaagccaagt
     3781 tttaaatact tatatagaat tgtactttgc acataccaaa ttatcaaaat gtcaaagaga
     3841 aagacacaga aaaagatcac tttgtccgaa agaagaaact cccatgcaac actaactgaa
     3901 atattttcgg cttctagagg tatctttgat tatgtcatta atacaataat gttatataag
     3961 caaacataaa ctttcttttc cttggaacct gtgcgacgag tatccaaaag aaaagcgaag
     4021 tggagtaact tctggccgct actctgccgt cattccccgt tctcggcaat tcgaccagcg
     4081 catttccggg ccaacatatt cttcttgtcg cgccaacttt ccgcactcgc tcgcattctc
     4141 caggatcccc acaaactatt tgccagagac tgtgctgacg taagcacgct tgctactgtc
     4201 agacttttct tctctcgctt cagtgacgaa aacaaaagag gaatgaatat cttgccctca
     4261 catcaaagtg gaaatctctg gagcactctc agaacatgaa gaagctatta ctgtcttcac
     4321 catcaccaag gcagtaacat caagcacaga gtatgaacca ttggaaaaac tagtaggaat
     4381 gttcttagat cactacgtta taagctcagt ttacgtagtg tggctagcaa atcaagaatg
     4441 gactataaat ataattgaca atttttcact tcaaggattt tatgtgcatc tgttatttta
     4501 tctgtaaata tcaaattatt taacaataat tattaaaaat ggacattaac tacaaagaac
     4561 aacaagaatt tcaaaaagtc gttgaacaaa agcaaatgaa cgatttcatg cgtgtatgta
     4621 ctcagatttt taactcaatt taatatcata tttgaagata tagatacaga tatatatata
     4681 tatatatttt caaatataaa gactttgaat tttagttact aactttccat tttttatttc
     4741 ccattcatcc catctctttt ttttattatt tttatatagt tatacactaa cttagtcgac
     4801 ctgcaggcat gcaagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     4861 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     4921 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     4981 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     5041 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     5101 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     5161 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     5221 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     5281 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     5341 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     5401 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     5461 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     5521 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     5581 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     5641 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
     5701 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     5761 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     5821 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     5881 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     5941 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     6001 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     6061 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     6121 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     6181 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     6241 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     6301 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     6361 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     6421 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     6481 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     6541 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     6601 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     6661 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     6721 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     6781 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     6841 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     6901 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     6961 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta
     7021 taaaaatagg cgtatcacga ggccctttcg tc
//
